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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
01/03/2019 |
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Actualizado : |
15/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
HAMOND, C.; SILVEIRA, C.S.; BURONI, F.; SUANES, A.; NIEVES, C.; SALABERRY, X.; ARÁOZ, V.; COSTA, R.A. DA; RIVERO, R.; GIANNITTI, F.; ZARANTONELLI, L. |
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Afiliación : |
CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENCIA BURONI, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; ALEJANDRA SUANES, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; CECILIA NIEVES, Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.//Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, UruguaY.; XIMENA SALABERRY, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ALMEIDA DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO RIVERO, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//6Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.; Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Montevideo, Uruguay./Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
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Título : |
Leptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Transboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133 |
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DOI : |
10.1111/tbed.13133 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019. |
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Contenido : |
Abstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH |
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Palabras claves : |
ACUTE LEPTOSPIROSIS; ENFERMEDADES DE LOS RUMIANTES; MOLECULAR DIAGNOSTICS; MORTALITY; PLATAFORMA DE SALUD ANIMAL; SALUD ANIMAL; SHEEP. |
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Thesagro : |
OVEJA; URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02629naa a2200373 a 4500
001 1059551
005 2020-01-15
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/tbed.13133$2DOI
100 1 $aHAMOND, C.
245 $aLeptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019.
520 $aAbstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH
650 $aOVEJA
650 $aURUGUAY
653 $aACUTE LEPTOSPIROSIS
653 $aENFERMEDADES DE LOS RUMIANTES
653 $aMOLECULAR DIAGNOSTICS
653 $aMORTALITY
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSALUD ANIMAL
653 $aSHEEP
700 1 $aSILVEIRA, C.S.
700 1 $aBURONI, F.
700 1 $aSUANES, A.
700 1 $aNIEVES, C.
700 1 $aSALABERRY, X.
700 1 $aARÁOZ, V.
700 1 $aCOSTA, R.A. DA
700 1 $aRIVERO, R.
700 1 $aGIANNITTI, F.
700 1 $aZARANTONELLI, L.
773 $tTransboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/04/2018 |
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Actualizado : |
25/04/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. |
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Afiliación : |
JUAN P. GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA L. FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía; SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS |
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DOI : |
10.3389/fpls.2018.00262 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018. |
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Contenido : |
ABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress.
© 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal. MenosABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (Gm... Presentar Todo |
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Palabras claves : |
ABIOTIC STRESS; ARABIDOPSIS; DROUGHT; EIFiso4G; SOYBEAN CROP; TRANSLATION INITIATION. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9385/1/Frontiers-in-Plant-Science.-2018.fpls-09-00262.pdf
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Marc : |
LEADER 03453naa a2200289 a 4500
001 1058480
005 2018-04-25
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2018.00262$2DOI
100 1 $aGALLINO, J.P.
245 $aA dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.
520 $aABSTRACT. Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress. © 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal.
653 $aABIOTIC STRESS
653 $aARABIDOPSIS
653 $aDROUGHT
653 $aEIFiso4G
653 $aSOYBEAN CROP
653 $aTRANSLATION INITIATION
700 1 $aRUIBAL, C.
700 1 $aCASARETTO, E.
700 1 $aFLEITAS, A.L.
700 1 $aBONNECARRERE, V.
700 1 $aBORSANI, O.
700 1 $aVIDAL, S.
773 $tFrontiers in Plant Science, 2018$gv.9, Article number 262. (2 March 2018). OPEN ACCESS
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